Isolation of Glycosylated Flavonoid from Ruta graveolens and study its effect on Hepatocellular Carcinoma

Abstract

Background: A large number of natural compounds have been demonstrated to have cancer chemopreventive activity and healing due to their strong antioxidant and cytotoxic activities. Objective: The present study investigates the effect of encapsulation of rutin with carboxymethyl chitosan against N-Nitrosodiethylamine (Di-ethylnitrosamine) (DENA)-induced and carbon tetrachloride-promoted Hepatocellular carcinoma in male Wistar rats. Materials and Methods: Fifty male Wistar rats were divided into two major groups. Group I serves as control; Group II was induced HCC by DENA (200mg/kg b.wt) followed by carbon tetrachloride (3ml/kg subcutaneously) for 6 weeks. Group II was divided into four subgroups. Subgroup I (induced subgroup), which took DENA+CCL4, was only then injected with DMSO. Subgroup II (rutin subgroup) rats induced by DENA+CCL4 then injected with rutin at a dose of 20mg/kg b.w. I.p three times a week. Subgroup III (carboxymethyl chitosan subgroup) rats induced by DENA+CCL4 then injected with carboxymethyl chitosan at a dose of 13.5mg/kg b.w. I.p thee times a week. Subgroup IV (CMC+Rutin subgroup) rats were induced by DENA+CCL4, then injected with the combination at a dose of 16mg/kg b.w. I.P. three times a week. Results: Observed that there was a significant increase in liver marker enzymes (ALT P<0.001), (AST P<0.087), (ALP P=0.004) and (Albumin P=0.003), tumor marker enzymes (AFP, CEA at P<0.001), kidney function (creatinine P=0.001) and antioxidant activities have no significant values. Conclusion: The results suggested that encapsulation of rutin in carboxymethyl chitosan enhances the effect of rutin against tumor cells.